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1C; Table 1), a phenotype that was not observed in sqn-4, sqn-8, and sqn-9 when grown simultaneously for this experiment. However, the strength of the sqn phenotype varies depending on growth conditions, the general trend being that healthier, sturdier plants have a stronger phenotype. In different experiments, sqn-4 plants actually showed an enhanced phenotype, with indeterminate flowers (Fig. 1D, J). When the Col0 sqn alleles are ordered into an allelic series based on the severity of the flower phenotype, sqn-1 and sqn-5, which were inferred to be null alleles (Smith et al., 2009), are the strongest alleles, and sqn-8 and sqn-9 are the weakest alleles. With the notable exception of carpel number, the Ler allele sqn-4 appears intermediate, in terms of both floral organ numbers and indeterminacy. Yet, sqn-4 exhibits a stronger increase in carpel number than any other allele, including sqn-1 and sqn-5 (Fig. 1E), which may be an ecotype-specific effect, as the ERECTA gene was shown to control meristem size and WUS expression (Mandel et al., 2014). At the molecular level, sqn-1, sqn-4, and sqn-5 have point mutations or T-DNA insertions that generate early stop codons in the SQN mRNA, while sqn-8 and sqn-9 have T-DNA insertions in the SQN promoter (Supplementary Table S1 at JXB online). The SQN mRNA level appears reduced in sqn-1, sqn-4, and sqn-8, but, surprisingly, it is increased in sqn-5 and sqn-9 (Supplementary Fig. S1). However, the 3′ end of the SQN mRNA is missing in sqn-5 (Supplementary Fig Read more↗

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Content typeapplication/zip
Updated2 years ago
Checked5 months ago
Hostoup.silverchair-cdn.com
Keywordserv394 supplementary data zip erv
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